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ELISA試劑盒分析氨肽酶A抗體報(bào)告
發(fā)布時(shí)間： 2014-04-16 點(diǎn)擊次數(shù)： 1266次ELISA試劑盒試 劑 組 成
1、精密度微孔板Microtest Plates 96 wells
2、酶標(biāo)偶合液Enzyme Conjugate 12.0ml
3、標(biāo)準(zhǔn)品Standard 5 x 1.0ml
4、呈色劑A、Substrate A 6.0ml
5、呈色劑B、Substrate B 6.0ml
6、終止液Stop Solution 6.0ml
7、濃縮洗滌液（1：20）Rinsing Buffer 60.0ml
8、5倍樣品稀釋液 dilution 15.0m
英文名稱 Anti-Aminopeptidase A/BP1/CD249
中文名稱 氨肽酶A抗體
別 名 Aminopeptidase A; AMPE_HUMAN; AP-A; APA; CD249; Differentiation antigen gp160; EAP; EC 3.4.11.7; ENPEP; Glutamyl aminopeptidase; Gp160; Ly51; Ly51/6C3 antigen.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Cow, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 發(fā)育生物學(xué) 干細(xì)胞 淋巴細(xì)胞 b-淋巴細(xì)胞
蛋白分子量 predicted molecular weight: 109kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Aminopeptidase A
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
ELISA試劑盒氨肽酶A抗體產(chǎn)品介紹 Aminopeptidase A, also designated APA, gp160 human kidney differentiation antigen, glutamyl aminopeptidase, or enpep, is a differentiation-related kidney glycoprotein. As a cell surface, zinc-dependent metalloprotease, Aminopepti-dase A specifically cleaves amino-terminal acidic residues from peptide substrates such as Angiotensin II. APA is expressed on the surface of epithelial cells of the glomerulus and proximal tubule cells of the human nephron, where it may mediate the constitutive trafficking of Glut4-containing vesicles. These Glut4-containing vesicles are tissue-specific secretory-like microsomal structures that mediate insulin-dependent translocation of GLUT4 to the cell surface in fat and muscle cells. Mutations in the gp160/APA gene, including loss of protein expression or enzymatic activity, occur in 20% of primary clear cell renal carcinomas.
Function : Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Subcellular Location : Membrane.
Tissue Specificity : Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Similarity : Belongs to the peptidase M1 family.
Database links : UniProtKB/Swiss-Prot: Q07075.3